Secoll™ Lymphocyte separation solution is made with Polysucrose 400 (PS400). PS400 is a hydrophilic polymer with a molecular weight of 400000 Dalton. It is used for the production of density gradients for the separation of cells and sub-cellular components, which sediment during centrifugation due to gravity. Our Red Blood Cell Lysis Buffer is a sugar-based buffer whose formulation has been optimized for the preferential lysis of red blood cells from human whole blood, while having minimal effect of leukocytes, including lymphocytes. As Sugars at high temperature can cause caramelization (browning), which degrades the sugars, therefore autoclaving is not the best option for sterilization of such products. Therefore, our RCL products are sterilized using pharmaceutical-grade filtration.