DMEM/F12 Medium is a mixture of DMEM and Ham’s F-12 and is a superior rich and complex medium. DMEM/F12 delivers greater reliability, performance, and improved control in mammalian cell culture. DMEM/F-12 is a widely used basal medium for supporting the growth of many different mammalian cells including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. A 1:1 mixture of DMEM and Ham's F-12 is the most-used formulation. HEPES buffer is included in the formulation at a final concentration of 15 mM to compensate for the loss of buffering capacity incurred by eliminating se