Versene is a Non Animal Origin chelating agent that deataches the cells by the below processes: - Chelation of Calcium Ions: EDTA in Versene binds to calcium ions in the culture medium. Calcium is essential for cell adhesion molecules, such as cadherins, which help cells stick to each other and to the culture vessel. - Disruption of Cell Adhesion: By chelating calcium, Versene disrupts these cell adhesion molecules, causing cells to detach from the culture surface and from each other. - Gentle Cell Dissociation: Unlike enzymatic methods (e.g., trypsin), Versene provides a gentler way to dissociate cells, reducing cell damage and promoting higher cell viability. This makes Versene particularly useful for routine passaging of sensitive cell types, such as human pluripotent stem cells (hPSCs), where maintaining cell viability and integrity is crucial.