01

Microbiology/Virology

Viral Transport Medium (VTM), allows the safe transfer of viruses, chlamydiae, and mycoplasma for further research including conventional cell culture methods, diagnostic tests, and molecular biology techniques. Our product contains heat-inactivated Fetal Bovine Serum, Gentamycin and Amphotericin B. The composition and manufacturing of our VTM follows the official Center for Disease Control and Prevention (CDC) guidelines. Serana‘s VTM is also offered in various bulk sized aliquots (5L, 10L).

02

Cytogenetics and Supplements

Colcemid Solution: for chromosome analysis during lymphocyte karyotyping and amniotic fluid cell chromosome analysis, and in cell synchronization. Potassium Chloride: for preparation of blood lymphocyte chromosomes. Sodium Citrate: is a hypotonic solution used for chromosome karyotyping. Trypsin EDTA Solution: is used for chromosome digestion before staining (Giemsa banding). Lymphogrow: is a medium has been specifically optimised for short-term culture of peripheral blood lymphocytes for chromosome analysis. Amniogrow: designed for the primary culture of human amniotic fluid cells and chorionic villi (CV) cells.

03

Separation Solutions

Secoll™ Lymphocyte separation solution is made with Polysucrose 400 (PS400). PS400 is a hydrophilic polymer with a molecular weight of 400000 Dalton. It is used for the production of density gradients for the separation of cells and sub-cellular components, which sediment during centrifugation due to gravity. Our Red Blood Cell Lysis Buffer is a sugar-based buffer whose formulation has been optimized for the preferential lysis of red blood cells from human whole blood, while having minimal effect of leukocytes, including lymphocytes. As Sugars at high temperature can cause caramelization (browning), which degrades the sugars, therefore autoclaving is not the best option for sterilization of such products. Therefore, our RCL products are sterilized using pharmaceutical-grade filtration.

04

Cryopreservation Media

The reduction of biological and biochemical processes at low temperatures can generate a possibility for the long-term preservation of cells and tissues. However, ice crystals cause freezing death penalty for almost all living cells. Water-to-ice transition is the critical phase for cells counteracting low temperatures. In cryopreservation process, presence of a Cryoprotective Agent (CPA) affects the rate of water transport, nucleation, and crystallization in a way that leads to the maintenance of the cell structure and bringing the biological sample in a suspended animation status at cryogenic temperature for any considerable period of time. The cryopreservation media containing CPA is one of the critical components of a fruitful cryopreservation. Depending on the cryopreservation procedure and targeted tissue/cells media can differ.